Metagenomic insights into the response of indigenous microbial communities in beach sands to the Deepwater Horizon oil spill

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doi: 10.1180/minmag.2013.077.5.11
Authors:Kostka, J. E.; Rodriguez-R, L. M.; Overholt, W. A.; Lin, X.; Marks, K.; Konstantinidis, K.; Huettel, M.
Author Affiliations:Primary:
Georgia Institute of Technology, Atlanta, GA, United States
Other:
Florida State University, United States
Volume Title:Goldschmidt abstracts 2013
Source:Mineralogical Magazine, 77(5), p.1501; Goldschmidt 2013, Florence, Italy, Aug. 25-30, 2013. Publisher: Mineralogical Society, London, United Kingdom. ISSN: 0026-461X
Publication Date:2013
Note:In English
Summary:Biodegradation mediated by indigenous microbial communities is the ultimate fate of the majority of hydrocarbons that enter the marine environment. A large amount of oil from the Deepwater Horizon oil spill was transported to and subsequently buried in Gulf of Mexico beaches. The objective of this research is to characterize the in situ response of microbial communities in parallel with the fate and chemical changes in oil hydrocarbons. Our time series database encompasses >500 sediment and water samples collected from Pensacola Beach, FL, USA, from 2010 to 2012. Illumina MiSeq and HiSeq platforms were used to obtain an average of 10000 SSU rRNA gene amplicon sequences and 35 million (100 bp) paired-end reads on a subset of these samples. A bloom of bacteria was observed in parallel with oxygen consumption rates and the depletion of the majority of highly degradable oil hydrocarbons during the first 4 months after oil came ashore. SSU rRNA gene amplicon and metagenome analysis revealed an initial sharp drop in community diversity after oiling. Moreover, evidence of succession was detected across the time-series, featuring a marked increase in relative abundance of Alcanivorax spp. during the first two months (up to 40% of the community) followed by enrichment in other members of the Gammaproteobacteria (Parvularcula, Hyphomonas, Xanthomonadales) in subsequent months. By July 2011, community diversity had sharply rebounded, and sequences of taxa associated with oligotrophic conditions, such as the Thaumarchaeota (Nitrosopumilus) were detectable, whereas phylotypes associated with the oiling event were undetectable. Finally, we observed that genes for central-metabolism functions were enriched in clean samples, while genes related to peripheral metabolism (e.g. aromatics degradation, nitrogen fixation, phosphorus uptake) were enriched in oiled samples, reflecting the community response to a oil-derived carbon source and nutrient depletion. Overall, multiple lines of independent evidence from a time series describe the microbial response to hydrocarbon discharge as a succession dynamics model driven by nutrient availability and hydrocarbon chemistry.
Subjects:Bioavailability; Biodegradation; Carbon; Clastic sediments; Coastal environment; Communities; Deepwater Horizon oil spill; Hydrocarbons; Nitrogen; Nucleic acids; Nutrients; Oil spills; Organic compounds; Phosphorus; Pollution; RNA; Samples; Sand; Sediments; Statistical analysis; Time series analysis; Water; Atlantic Ocean; Escambia County Florida; Florida; Gulf Coastal Plain; Gulf of Mexico; North America; North Atlantic; United States; Alcanivorax; Bacteria; Gammaproteobacteria; Hyphomonas; Nitrosopumilis; Parvularcula; Pensacola Beach Florida; Xanthomondales
Coordinates:N301500 N310000 W0871300 W0875500
Record ID:2014015985
Copyright Information:GeoRef, Copyright 2018 American Geosciences Institute. Abstract, Copyright, Mineralogical Society of Great Britain and Ireland
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